"OASIS Implant" Deep-Brain Imaging & Stimulation System for Freely Behaving Animals

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OASIS Implant a ground-breaking system that is capable of performing simultaneous all-optical recording (e.g. Calcium imaging) and manipulation (e.g. optogenetic stimulation) in the deep brain of a freely behaving animal to observe neural circuit activity with high cellular resolution. Compatible with high-sensitivity research-grade cameras, OASIS Implant enables researchers to acquire high quality deep-brain images with low noise and high linearity, enabling quantitative data analysis. In addition, with multiple illumination ports and multiple filter slots, OASIS Implant can be reconfigured by end users to precisely fit their specific experimental needs. Equipped with both wide-field and patterned illumination capabilities (when combined with Mightex's market-leading Polygon400), OASIS Implant allows scientists to simultaneously perform Calcium imaging and targeted optogenetic stimulation with pin-point accuracy to stimulate individual neurons (but without unwanted stimulation of the neighbouring neurons). Moreover, it is also capable of multi-color imaging and optogenetic stimulation. Another key benefit of the OASIS Implant system for behavioral neuroscience is its super light-weight head mount. Since both deep-brain imaging and stimulation are delivered through a very thin imaging fiber, the overall weight of the head-mounted fixture is as little as 0.7g (compared to approximately 2~3g with a head-mounted camera), which minimizes stress experienced by the animal, and allows for the duration of the experiments to be extended. All of these features combined make the OASIS Implant an ideal research tool for in vivo experiments.

In addition to many other applications, OASIS Implant enables bioscientists to do the following:

1. Deep brain calcium imaging in freely behaving animals;

2. Deep brain patterned optogenetic stimulation with single-cell or sub-cellular resolution; and

3. Simultaneous deep brain stimulation and imaging.

One Platform, Any Experiment



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Key Features

  • In vivo all-optical recording and manipulatiion of neural circuit activity;
  • Simultaneous Calcium imaging and optogenetic stimulation;
  • Sub-cellular resolution, high-quality imaging for quantitative analysis;
  • Multi-wavelength illumination;
  • Patterned stimulation with sub-cellular targeting capability;
  • Super-light head mounted fixture, 0.7g;
  • Modular system design, reconfigurable for different applications.

 

 

Superior Deep-Brain Imaging Capability

 

The OASIS Implant provides single-cell and population resolution imaging for a wide-range of experimental setups. With a standard c-mount camera port, OASIS Implant accepts any cameras, including research-grade scientific cameras which enable scientists to capture high-quality deep-brain images and videos. Furthermore, the OASIS Implant is compatible with multiple light sources to image different imaging indicators and fluorophores. Thus, the OASIS Implant will enable scientists to acquire more detailed and more accurate data for not only qualitative, but also quantitative analysis.

GCaMP6 imaging in the Striatum using the OASIS Implant with a 300um imaging fiber.

 
Deep Brain Wide-field and Targeted Optogenetic Stimulation

The Imaging Fiber Probe (IFP) consists of thousands of individual mini-fibers or pixels, and each of these pixels can be independently addressed with Miughtex's market-leading Polygon400 patterned illuminator. Therefore, the OASIS Implant can be used to deliver light to deep brain regions with sub-cellular resolution, and this opens the door for scientists to conduct experiments, such as deep-brain sub-cellular optogenetic stimulation or targeted calcium imaging, in a freely-moving animal. In addition, traditional wide-field illumination can easily be supported by sending light through all the individual pixels in the IFP. The OASIS Implant platform has two illumination ports, each capable of taking either a wide-field or a patterned illuminator with single or multiple wavelengths/colors.

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The Polygon400 patterned illuminator can be used to direct light through a single fiber, group of fiber, or multiple groups of fibers for high cellular stimulation resolution in vivo.

 
Freely-Moving Capabilities

The OASIS Implant enables imaging and stimulation in a freely-moving animal. This is due, in part, to the light-weight headmount (<0.7g) and the system being external from the animal. These requirements will help extend the length of experiments and reduce stress for imaging and stimulation experiments.

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The light headmount required to use the OASIS implant enables a rodent to be freely-moving.   The headmount for the OASIS Implant.


Data-Analysis to Study Large-Scale Neural Dynamics

With included software, users can acquire a continuous stream of data from within the deep brain to identify and define neurons, and then extract intensity traces for each cell. Calcium traces can also be synchronized and time-stamped with other behavioural equipment (cameras, behavioural equipment etc.) to generate precise and comprehensive data for analysis.

GCaMP imaging of neuronal activity in striatum of free- moving mouse   Fluorescence tracing on selected region of interest



OASIS Implant System Schematic

 

As shown in the schematic diagram below, a typical OASIS Implant system contains the following parts:

(1) At the heart of the system, an OASIS Implant platform that connects all the parts together;

(2) An Imaging Fiber Probe (IFP, also called "Fiber Bundle") that consists of thousands of mini-fibers, each of which can be independently addressed. The IFP is used both for delivering images from the live animal to the OASIS Implant platform, and for delivering light/stimulation from the OASIS Implant platform to the live animal;

(3) A GRIN Lens implant which will be placed into the brain of the live animal;

(4) A Fiber Mount which is used to connect the GRIN lens and the IFP;

(5) A Scientific Camera which is mounted onto the C-mount on the OASIS Implant platform. Images from the deep brain are delivered to the camera by the IFP through the OASIS Implant platform;

(6) Up to two Wide-Field and/or Patterned Illumination Light Sources (such as Mightex's Polygon400) that can be inserted into the OASIS Implant platform. The light from the light sources is delivered to the live animal through the IFP; and

(7) Light Source Controllers (e.g. LED controllers etc) that are used to control the wide-field and/or the patterned illumination light sources.





OASIS Implant supports both uniform wide-field illumination and patterned illumination when used with a Polygon400 patterned illuminator. The versatility of the OASIS Implant allows researcher to use both illumination methods simultaneously. One example is to use patterned illumination to optogenetically stimulate selected neurons while observing fluorescence images with the wide-field illumination. Another example is to use structured illumination from the patterned illuminator to suppress background during fluorescence imaging.

 

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