PolyScan Optogenetic Patterned Stimulation Software

 

 

For operation of all Mightex Bioscience Equipment including the Polygon400 Patterned Illuminator and OASIS modular micro and macroscope systems.

The PolyScan software platform is an integrated control center for all your Mightex equipment including the Polygon400 patterned illuminator, CCD cameras and LED controllers and is designed to help microscopy and bioscience researchers execute sophisticated illumination and imaging experiments.

PolyScan is able to meet the demands required of common bioscience illumination experiments such as optogenetics, photo-uncaging, and imaging because of the feedback from leading researchers in these fields, and the years of expertise within the Mightex software team. Please read below for more information about the key features and tools within PolyScan or contact us for information about how PolyScan can be used for your research.

 

 

Integrated Software Control Center

Operate all Mightex Bioscience Equipment in a Single, Easy to Use Platform
PolyScan is an all in one software platform to control all Mightex bioscience equipment and research tools. Supported devices include the Polygon400 patterned illuminator, LED drivers, digital/analog control boxes and CCD microscopy cameras.

Hardware_configuration



Calibration Tool to Match the Polygon400 Illumination Area with the Viewing Area of Any 3rd Party Camera
The 3rd party camera calibration tool allows users to synchronize and match the illumination field of view of the Polygon400 patterned illuminator with the imaging area of any 3rd party camera with a viewing window.

Calibration_wizard

 

Spatial Illumination Pattern Definition & Arrangement

Easy to Use Pattern Creator
The pattern drawing feature provides users with a powerful and easy to use tool to create custom, user defined patterns to be projected by the Polygon400 onto a target. The GUI allows for any shape(s) to be generated from simple squares, circles and lines to more complex arbitrary shapes such as a ring, or pattern that matches the shape of a given cell body.

Pattern_editor



True Dimension Illumination Feature Sets the Physical Size of Illumination Patterns
This feature allows users to not only create custom illumination patterns, but also define the physical size of the illumination pattern as it will appear on their sample. For example, this could be a 100um x 150um rectangle a 25 um diameter circle, or a 50um long line.

True_dimension



Pattern Sequence Construction & Editing for Provides Flexibility and Organization
PolyScan allows users to arrange their custom defined patterns in a specific order for their experiments. The GUI provides a helpful overview of all the current patterns in the sequence, and their ordering while allowing the user to edit and re-arrange their patterns. New features include merging patterns and splicing a single pattern with several elements into multiple patterns.

Profile_sequence_editor



Control Temporal Parameters with the Integrated Waveform Editor
New to PolyScan is the integrated waveform editor. This allows users to define the intensity and temporal properties of the illumination. This includes everything from simple square pulses with a defined duration to more complex waveforms such as a ramp-up intensity plot or arbitrary waveforms.

Waveform_editor



Profile Builder for Defining 5 Dimensions of Illumination Control
The Profile builder mode allows users to assign each 2D spatial pattern they create with a custom waveform (1D in time and 1D in intensity), and a wavelength. This means that the user can define pattern 1 to be a circle using blue light pulsed at 50% intensity for 10ms and pattern 2 to be a square pattern with UV light pulsed 5 times, each pulse at 5ms, and ramping up intensity by 10% step sizes. This can be done with every pattern in the sequence.

Profile_sequence_editor2



Perform Grid Scanning & Path Scanning Experiments
Users can generate advance grid scanning or path scanning sequences in only a couple steps. In grid scanning PolyScan will automatically generate a sequence that sequentially or pseudo-randomly scans across the full area of the Polygon field of view. This is done by dividing the area into a grid of any size from 1x1 to 10x10. With the Path Scanning feature the user can automatically generate a sequence where a custom pattern will scan along a path (e.g. a line, circle or arbitrary path). PolyScan does this (1) draw an arbitrary path (2) draw any pattern and (3) specifying the amount of intervals. Then a sequence will automatically be generated.

Grid_scanning



Import Vector Files as Illumination Patterns
For users who choose to use MatLab or LabView to generate grid scanning scripts for their patterned illumination sequences. These scripts can be easily imported into PolyScan as vector files.

Vector File Sequences


 
Define and Specify Light Controlled Experiments

Centralize Experiment Management with the Session Control
PolyScan’s Session Control provides users with a wide range of tools to help them execute a wide variety of different patterned illumination experiments using the profile sequences they generated. Session control provides the user with access to operate the Polygon in “master” or “slave” mode, set timing parameters, and also specify key synchronization details such as trigger delay, or trigger pulse width. In addition, the user can also control the timing parameters of each pattern with the built-in LED controller feature.

Session_control



Built-in Session Notebook to Log All Experimental Information
The Notebook feature allows users to log key experimental information about their experimental set-up, specimen, and parameters of any integrated equipment. The notebook will also automatically link to the illumination profile sequence that was upload and even allow users to take image snapshots of their sample.

Session_notebook


 

Ordering Information:
Model Name Price Buy Now Availability
Product specifications are subject to change without prior notice. All prices are FOB California, unless otherwise stated.
Please call/email for volume pricing.
For sales and customer support:

Please call (USA) +1-925-218-1885 or (Canada) +1-416-840-4991,
or email sales@mightexsystems.com or sales@mightex.com.