OASIS In Vivo Macro & Microscope Platform


OASIS

Optical Activation, Stimulation & Imaging System
 

BRAIN Initiative: Targeted BRAIN Circuits Project (R01 Grant) 2017.

Customer Reference: OASIS modular system for live animal optogenetic stimulation.

Researchers at NYU use Mightex's OASIS to study spatio-temporal codes in the olfactory bulb of awake and behaving mice.

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White Paper: Patterned Illumination Systems for Optogenetics

 



Translates and Rotates for Live Animal Research

Simultaneously Image & Deliver Wide-field and/or Pattern Illumination

Macro or Microscopic Optical Assembly

Modular Platform Tailors to any Experimental Application

Excellent System for:
    In Vivo Optogenetics
    Photostimulation
    Photoactivation

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Mightex's Optical Activation, Stimulation and Imaging System (OASIS) is designed specifically to meet the growing demand to conduct experiments on live animals, when system flexibility is a primary consideration and a large Field of View (FoV) is usually required. In Optogenetics, for example, in vivo patterned illumination on rodents, monkeys and other animals is the next step towards the ultimate goal of being able to record and control the activity of each neuron in the brain of behaving animals at a single cell and ms resolution. To achieve patterned illumination for in vivo applications, the OASIS features Mightex’s market-leadingPolygon400 patterned Illuminator to deliver light to a specified area of the specimen while leaving neighboring areas untouched.

Whereas traditional microscopes are a fixed system with limited flexibility, OASIS is designed to provide excellent reconfigurability/flexibility and can be configured to work around a fixed specimen such as a sedated live animal. A heavy-duty precision XYZ stage is mounted on the support structure and the OASIS optical assembly is in turn mounted on the XYZ stage. The XYZ stage controls the lateral motion as well as focus of the system. A pan/tilt mechanism add-on is also available to orient the OASIS relative to the live animal. Motorized versions of both the XYZ stage and tilt mechanism are available.

 

 

OASIS Overview

OASIS is a modularized imaging & illumination system that was designed to address the challenges existing In Vivo and In Vitro research. In particular, OASIS is a system that can configure to move and tilt to a fixed specimen while mainting excellent optical efficiency found in commercial microscopes and imagining system.

Because the design of OASIS is a modular one, users have the opportunity to customize OASIS to perfectly match the requirements of their application. This can include, but is not limited to selecting a microscope vs macroscope design, multiple patterned illumination devices, dual patterned and wide-field illumination, dual camera options, as well as motorized stages and tilt mechanisms.

Bellow is a schematic illustrating the various sub-system components in OASIS. Scroll along the tabs above to read more information about each sub-section and to see the different customization options available for each sub-section. System level pictures of both the micro & macro versions of OASIS can be found at the bottom of this page.


OASIS Schematic (translation stage)


OASIS Schematic (stereotactic stage)


Example Systems

OASIS Macro OASIS Micro
OASIS Macro is designed for low magnification applications that require a large field of fiew (up to 4.5 x 8.2 mm). With a wider beam path and infinity corrected optics the macro-imaging system is able to take advantage of low-mag high NA macro objectives. The result is higher illumination intensity covering a larger area when comparing to a commercial microscope. Imaging sensitivity is also dramatically improved because of the high NA objective that can collect more fluorescence signal from the specimen. The OASIS micro-imaging system features infinity-corrected optics and accepts common microscope objective lenses. This system will provide a field of view, and output intensity from the Polygon that is similar to when using a commercial microscope. Therefore, It is recommended for high resolution application similar to that of typical commercial microscopes.

OASIS Macro with Stereotactic Stage, Two-deck optical assembly
This OASIS Macro system features infinity-corrected optics, stereotactic stage (X-Y-Z translation, pitch and yaw) and a two-deck optical assembly to accept two light sources (e.g. one patterned illuminator and one wide-field illuminator). OASIS Macro is designed for low magnification applications that require a large Field of View (FoV). With a wider beam path and infinity corrected optics the macro-imaging system is able to take advantage of low-magnification and high NA macro objectives. The result is higher illumination intensity covering a larger area when comparing to a commercial microscope. Imaging sensitivity is also dramatically improved because of the high NA objective that can collect more fluorescence signal from the specimen.

 

 

Ordering Information:
Model Name Price Buy Now Availability
Product specifications are subject to change without prior notice. All prices are FOB California, unless otherwise stated.
Please call/email for volume pricing.
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Please call (USA) +1-925-218-1885 or (Canada) +1-416-840-4991,
or email sales@mightexsystems.com or sales@mightex.com.