"OASIS Implant" Deep-Brain Optogenetic System for Freely Behaving Animals

 

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OASIS Implant a ground-breaking system that is capable of doing deep-brain simultaneous all-optical recording (e.g. Calcium imaging) and manipulation (e.g. optogenetic stimulation) of neural circuit activity with cellular resolution, on freely behaving animals. Compatible with high-sensitivity research-grade cameras, OASIS Implant enables researchers to acquire high quality deep-brain images with low noise and high linearity, enabling quantitative data analysis. In addition, with multiple illumination ports and multiple filter slots, OASIS Implant can be reconfigured by end users who can tailor make the system to fit their specific experimental needs. Equipped with both wide-field and patterned illumination capabilities (when combined with Mightex's market-leading Polygon400), OASIS Implant allows scientists to do simultaneous Calcium imaging and targeted optogenetic stimulation with cellular resolution. Moreover, it is also capable of doing multi-wavelength imaging/stimulation. More importantly, since both the deep-brain imaging and the deep-brain stimulation are delivered through an imaging fiber bundle, the overall weight of the head-mounted fixture is as little as 0.7g (compared to approximately 2~3g with a head-mounted camera), making OASIS Implant an ideal research tool for in vivo live animal experiments.

In addition to many other applications, OASIS Implant enables bioscientists to do the following:

1.      Deep brain calcium imaging in freely behaving animals;

2.      Deep brain patterned optogenetic stimulation with single-cell or sub-cellular resolution; and

3.      Simultaneous deep brain stimulation and imaging.

Below are a couple of deep-brain GCaMP imaging videos acquired using the OASIS Implant system in the striatum of a live and awake mouse. (Videos and recordings provided courtesy of academic neuroscience researchers.)

Key Features

  • In vivo all-optical recording and manipulatiion of neural circuit activity;
  • Simultaneous Calcium imaging and optogenetic stimulation;
  • Sub-cellular resolution, high-quality imaging for quantitative analysis;
  • Multi-wavelength illumination;
  • Patterned stimulation with sub-cellular targeting capability;
  • Super-light head mounted fixture, 0.7g;
  • Modular system design, reconfigurable for different applications.

 

 

 

Deep Brain Calcium Imaging and Fluorescence Tracing  


GCaMP imaging of neuronal activity in striatum of free-moving mouse

Fluorescence tracing on selected region of interest

 

OASIS Implant System Schematic

As shown in the schematic diagram below, a typical OASIS Implant system contains the following parts:

(1) At the heart of the system, an OASIS Implant platform that connects all the parts together;

(2) An Imaging Fiber Probe (IFP, also called "Fiber Bundle") that consists of thousands of mini-fibers, each of which can be independently addressed. The IFP is used both for delivering images from the live animal to the OASIS Implant platform, and for delivering light/stimulation from the OASIS Implant platform to the live animal;

(3) A GRIN Lens implant which will be placed into the brain of the live animal;

(4) A Fiber Mount which is used to connect the GRIN lens and the IFP;

(5) A Scientific Camera which is mounted onto the C-mount on the OASIS Implant platform. Images from the deep brain are delivered to the camera by the IFP through the OASIS Implant platform;

(6) Up to two Wide-Field and/or Patterned Illumination Light Sources (such as Mightex's Polygon400) that can be inserted into the OASIS Implant platform. The light from the light sources is delivered to the live animal through the IFP; and

(7) Light Source Controllers (e.g. LED controllers etc) that are used to control the wide-field and/or the patterned illumination light sources.

imaging_samples
 
OASIS Implant supports both uniform wide-field illumination and patterned illumination when used with a Polygon400 patterned illuminator. The versatility of the OASIS Implant allows researcher to use both illumination methods simultaneously. One example is to use patterned illumination to optogenetically stimulate selected neurons while observing fluorescence images with the wide-field illumination. Another example is to use structured illumination from the patterned illuminator to suppress background during fluorescence imaging.

 

Superior Deep Brain Imaging

With a standard C-Mount camera port, OASIS Implant accepts any cameras, including research-grade scientific cameras which enable scientists to capture high-quality deep-brain images and videos. In comparison to a head-mounted camera which usually contain a relatively low-grade CMOS image sensor, the image sensor used in a scientific camera has much better linearity and much higher sensitivity, which means that OASIS Implant will enable scientists to acquire more detailed and more accurate data for not only qualitative but also quantitative analysis.

imaging_samples
 



Deep Brain Wide-Field and Patterned Stimulation

The Imaging Fiber Probe (IFP) consists of thousands of individual mini-fibers or pixels, and each of these pixels can be addressed independently with Mightex's market-leading Polygon400 patterned illuminator. Therefore, the OASIS Implant can be used to deliver light to deep brain regions with sub-cellular resolution, and this opens the door for scientists to conduct experiments which otherwise impossible, such as deep-brain sub-cellular optogenetic stimulation or targeted calcium imaging in live animals. In addition, traditional wide-field illumination can easily be supported by sending light through all the individual pixels in the IFP.

OASIS Implant platform has two illumination ports, each capable of taking either a wide-field or a patterned illuminator with single or multiple wavelengths/colors.

imaging_samples
 
Additional Deep Brain Sample Videos


 

 

 

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