Polygon Patterned Illuminator Selection and Configuration Guide

 

1. Polygon400 Video Introduction

 

2. Polygon400 Series Selection Guide

  Standard Polygon400 Dense Pixel Polygon400 (DP) Fiber-coupled Polygon400 (DL)
  Polygon400 E-Series Polygon400 G-Series
Target Applications    • Optogenetics
   • Photoactivation
   • Photostimulation
   • Photopatterning
   • Calcium imaging
   • Optogenetics
   • Photoactivation
   • Photostimulation
   • Photopatterning
   • Calcium imaging
   • Uncaging
   • Photobleaching
   • Sectioning and Super
     Resolution Microscopy
   • Optogenetics
   • Photoactivation
   • Photostimulation
   • Photopatterning
   • Calcium imaging
   • High intensity applications
   • Large FoV applications
Wavelength Range Visible/NIR
(optimized for 400~700nm)
Visible/NIR
(optimized for 400~700nm)
UV/Visible/NIR
(optimized for 350~700nm)
Visible/NIR
(optimized for 400~700nm)
Light Sources Built-in LEDs only LED, Xenon, Arc lamp,
Halogen lamp, etc
LED, Xenon, Arc lamp,
Halogen lamp, etc
Laser, LED, Xenon, Arc lamp,
Halogen lamp, etc
Optical Interface N.A.
(with built-in LEDs)
3mm-core lightguide
(with 5mm ferrule)
3mm-core lightguide
(with 5mm ferrule)
Optical fiber
Projection Area Large
(e.g. 0.8mm x 1.4mm @10x)
Large
(e.g. 0.8mm x 1.4mm @10x)
Reduced
(e.g. 0.4mm x 0.7mm @10x)
Large
(e.g. 0.9mm diameter @10x)
Resolution High
(e.g. 1.6μm @10x, and 0.4μm @40x)
High
(e.g. 1.6μm @10x, and 0.4μm @40x)
Super-high
(e.g. 0.8μm @10x, and 0.2μm @40x)
High
(e.g. 1.8μm @10x, and 0.5μm @40x)
Light Intensity Under Microscope *** *** **** *****
Microscope Coupling Options Infinity Path
C-mount (camera port)
Infinity Path
C-mount (camera port)
Infinity Path Infinity Path
Software Compatibility PolyScan,
MicroManager,
NeuroPG,
NIS Elements (pending)
PolyScan,
MicroManager,
NeuroPG,
NIS Elements
PolyScan,
MicroManager(pending),
NIS Elements (pending)
PolyScan,
MicroManager(pending),
NIS Elements (pending)
All series supports the following features
Compatible with Electro-physiology & Optogenetic
Accepts TTL Input Trigger
Outputs TTL Signal for Synchronization
Analog Voltage Control Option1
Supports Arbitrary Waveforms2
Supports Mapping between Spatial Patterns and LED Wavelengths3
Supports Mapping between Spatial Patterns and LED Waveforms3

1. When used with an external light source controller with analog-input control capability; 2. When used with an external light source controller that supports arbitrary waveforms. 3. When used with a Mightex PolyScan2-compatible LED controller or I/O module.


 

3. Polygon400 System Configuration Guide

Below is a Polygon Patterned Illuminator Product Guide Chart. For more Polygon related topics, please refer to the Polygon FAQ's here:


 

Below is a step-by-step guide for choosing your Polygon400 patterned illumination solution:


Step-1: Choose the right wavelength(s) and light source(s) according to your specific application.
For example, for optogenetic applications you may choose 470nm for ChR2 and 590nm for NphR activation.


Step-2: Determine whether a Polygon E-series or a G-series will work better for your application.
For those optical wavelengths that cannot be found in Mightex’s LED wavelength portfolio, a Polygon G-series should be chosen, and you should find your own external light sources (such as LED’s, lasers, a Xenon Lamp, or a Mercury Lamp etc) with the desired wavelengths. The light sources must be compatible with a 3mm-core and 5mm-ferrule lightguide.


If your desired wavelengths are among the LED’s that Mightex has to offer, you may either opt for a Polygon E-series (which has built-in LED’s) or choose a Polygon G-series (which requires an external light source, as described above). For more details about the differences between a Polygon E-series and a G-series, please read this link: http://www.mightexsystems.com/family_info.php?cPath=245_243&categories_id=243.


Step-3: Choose the appropriate microscope adapter for mounting the Polygon onto your specific microscope.
A Polygon patterned illuminator is compatible with an upright or an inverted microscope, and it can be used with any commercial microscopes including (but not limited to) Leica, Nikon, Olympus and Zeiss. For more details about microscope adapters, please refer to this page: http://www.mightexsystems.com/family_info.php?cPath=120_245_274_276&categories_id=276.

Polygon can also be mounted onto a standard C-mount camera port on a microscope.


Step-4: Choose the best LED controller solution that will meet your specific application needs.
Whether you have a Polygon G-series or an E-series, (if you are using LED’s, built-in or external) there is a need to choose the appropriate LED controller solution that is best for your target application. LED’s can be controlled in one or more of the following ways: manual, analog, TTL and/or software. For more details about how to choose the best LED controllers, please refer to this page: http://www.mightexsystems.com/family_info.php?cPath=120_283_328&categories_id=328.

 

 

 

Ordering Information:
Model Name Price Buy Now Availability
Product specifications are subject to change without prior notice. All prices are FOB California, unless otherwise stated.
Please call/email for volume pricing.
For sales and customer support:

Please call (USA) +1-925-218-1885 or (Canada) +1-416-840-4991,
or email sales@mightexsystems.com or sales@mightex.com.